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cd34 selection beads kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd34 selection beads kit
    (A) Sorting strategy for SE hi FL-HSCs from freshly isolated E13.5 FL used for scRNA-seq. (B) Unsupervised clustering of E13.5 SE hi FL-HSC scRNA-seq data in UMAP. (C) Heatmap of gene-set scores by cluster for genes associated with adult HSC dormancy, serial-engrafting HSCs, diapause, , chemokine signaling (WP_CHEMOKINE_SIGNALING_PATHWAY), and a <t>common</t> <t>stem-cell</t> dormancy state associated with lipid metabolism, or genes associated with activated HSC/MPP states including high output and multilineage signatures, , , Myc pathway activation (Hallmark Myc Target Genes V1, V2), and metabolic activity (WP_TCA_CYCLE, HALLMARK_OXIDATIVE_PHOSPHORYLATION, WP_PURINE_METABOLISM). (D) Gene-set expression heatmaps for dormant HSC signature genes and serial-engrafting HSC signature genes. , (E) Expression heatmaps for modules of co-regulated genes. Gene modules 1, 3, 4, and 7 are shown with representative genes identified in each module.
    Cd34 Selection Beads Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+selection+beads+kit/CD34+MicroBead+Kit+UltraPure%2C+human/pmc12587490-333-29-32
    Average 96 stars, based on 289 article reviews
    cd34 selection beads kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution"

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.116289

    (A) Sorting strategy for SE hi FL-HSCs from freshly isolated E13.5 FL used for scRNA-seq. (B) Unsupervised clustering of E13.5 SE hi FL-HSC scRNA-seq data in UMAP. (C) Heatmap of gene-set scores by cluster for genes associated with adult HSC dormancy, serial-engrafting HSCs, diapause, , chemokine signaling (WP_CHEMOKINE_SIGNALING_PATHWAY), and a common stem-cell dormancy state associated with lipid metabolism, or genes associated with activated HSC/MPP states including high output and multilineage signatures, , , Myc pathway activation (Hallmark Myc Target Genes V1, V2), and metabolic activity (WP_TCA_CYCLE, HALLMARK_OXIDATIVE_PHOSPHORYLATION, WP_PURINE_METABOLISM). (D) Gene-set expression heatmaps for dormant HSC signature genes and serial-engrafting HSC signature genes. , (E) Expression heatmaps for modules of co-regulated genes. Gene modules 1, 3, 4, and 7 are shown with representative genes identified in each module.
    Figure Legend Snippet: (A) Sorting strategy for SE hi FL-HSCs from freshly isolated E13.5 FL used for scRNA-seq. (B) Unsupervised clustering of E13.5 SE hi FL-HSC scRNA-seq data in UMAP. (C) Heatmap of gene-set scores by cluster for genes associated with adult HSC dormancy, serial-engrafting HSCs, diapause, , chemokine signaling (WP_CHEMOKINE_SIGNALING_PATHWAY), and a common stem-cell dormancy state associated with lipid metabolism, or genes associated with activated HSC/MPP states including high output and multilineage signatures, , , Myc pathway activation (Hallmark Myc Target Genes V1, V2), and metabolic activity (WP_TCA_CYCLE, HALLMARK_OXIDATIVE_PHOSPHORYLATION, WP_PURINE_METABOLISM). (D) Gene-set expression heatmaps for dormant HSC signature genes and serial-engrafting HSC signature genes. , (E) Expression heatmaps for modules of co-regulated genes. Gene modules 1, 3, 4, and 7 are shown with representative genes identified in each module.

    Techniques Used: Isolation, Activation Assay, Activity Assay, Phospho-proteomics, Expressing

    Related Articles

    Centrifugation:

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution
    Article Snippet: .. After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130–091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130–091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130–100-453) according to the manufacturer protocol. .. CD34-enriched cells were cryopreserved at a density of 1X10 6 /mL in freezing media (90% FBS/10% DMSO) or Cryo-stor CS 10 (Sigma, C2874).

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution.
    Article Snippet: .. After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130-091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130-091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130-100-453) according to the manufacturer protocol. .. CD34-enriched cells were cryopreserved at a density of 1X106/mL in freezing media (90% FBS/10% DMSO) or Cryo-stor CS 10 (Sigma, C2874).

    Magnetic Cell Separation:

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution
    Article Snippet: .. After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130–091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130–091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130–100-453) according to the manufacturer protocol. .. CD34-enriched cells were cryopreserved at a density of 1X10 6 /mL in freezing media (90% FBS/10% DMSO) or Cryo-stor CS 10 (Sigma, C2874).

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution.
    Article Snippet: .. After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130-091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130-091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130-100-453) according to the manufacturer protocol. .. CD34-enriched cells were cryopreserved at a density of 1X106/mL in freezing media (90% FBS/10% DMSO) or Cryo-stor CS 10 (Sigma, C2874).

    Selection:

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution
    Article Snippet: .. After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130–091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130–091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130–100-453) according to the manufacturer protocol. .. CD34-enriched cells were cryopreserved at a density of 1X10 6 /mL in freezing media (90% FBS/10% DMSO) or Cryo-stor CS 10 (Sigma, C2874).

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution.
    Article Snippet: .. After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130-091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130-091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130-100-453) according to the manufacturer protocol. .. CD34-enriched cells were cryopreserved at a density of 1X106/mL in freezing media (90% FBS/10% DMSO) or Cryo-stor CS 10 (Sigma, C2874).



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    Image Search Results


    (A) Sorting strategy for SE hi FL-HSCs from freshly isolated E13.5 FL used for scRNA-seq. (B) Unsupervised clustering of E13.5 SE hi FL-HSC scRNA-seq data in UMAP. (C) Heatmap of gene-set scores by cluster for genes associated with adult HSC dormancy, serial-engrafting HSCs, diapause, , chemokine signaling (WP_CHEMOKINE_SIGNALING_PATHWAY), and a common stem-cell dormancy state associated with lipid metabolism, or genes associated with activated HSC/MPP states including high output and multilineage signatures, , , Myc pathway activation (Hallmark Myc Target Genes V1, V2), and metabolic activity (WP_TCA_CYCLE, HALLMARK_OXIDATIVE_PHOSPHORYLATION, WP_PURINE_METABOLISM). (D) Gene-set expression heatmaps for dormant HSC signature genes and serial-engrafting HSC signature genes. , (E) Expression heatmaps for modules of co-regulated genes. Gene modules 1, 3, 4, and 7 are shown with representative genes identified in each module.

    Journal: Cell reports

    Article Title: Differentiation latency and dormancy signatures define fetal liver hematopoietic stem cells at single-cell resolution

    doi: 10.1016/j.celrep.2025.116289

    Figure Lengend Snippet: (A) Sorting strategy for SE hi FL-HSCs from freshly isolated E13.5 FL used for scRNA-seq. (B) Unsupervised clustering of E13.5 SE hi FL-HSC scRNA-seq data in UMAP. (C) Heatmap of gene-set scores by cluster for genes associated with adult HSC dormancy, serial-engrafting HSCs, diapause, , chemokine signaling (WP_CHEMOKINE_SIGNALING_PATHWAY), and a common stem-cell dormancy state associated with lipid metabolism, or genes associated with activated HSC/MPP states including high output and multilineage signatures, , , Myc pathway activation (Hallmark Myc Target Genes V1, V2), and metabolic activity (WP_TCA_CYCLE, HALLMARK_OXIDATIVE_PHOSPHORYLATION, WP_PURINE_METABOLISM). (D) Gene-set expression heatmaps for dormant HSC signature genes and serial-engrafting HSC signature genes. , (E) Expression heatmaps for modules of co-regulated genes. Gene modules 1, 3, 4, and 7 are shown with representative genes identified in each module.

    Article Snippet: After centrifugation, the pellet was resuspended in 4 mL MACs buffer (MACS BSA Stock Solution Miltenyi Biotec 130–091-376, in autoMACs Rinsing Solution, Miltenyi Biotec 130–091-222) for CD34 enrichment using CD34 selection beads/kit (Miltenyi Biotech, 130–100-453) according to the manufacturer protocol.

    Techniques: Isolation, Activation Assay, Activity Assay, Phospho-proteomics, Expressing